FlexLab
Multi-step F-18 radiotracer production lab
The FlexLab is the most versatile F-18 radiosynthesizer specifically suited to the research, development and fully automated production of multi-step radiotracers.
The FlexLab allows the radiochemist to fully automate the radiosynthesis of multi-step compounds thereby improving synthesis yields, simplifying daily production and most importantly reducing the operators radiation exposure by completely eliminating the need of preform any manual processes.
For complex multi step F-18 radiotracer research & development, the FlexLab has been invaluable
Dr. Uwe Ackermann
Chief Research Radiochemist
Austin Hospital, Australia
Open Up New Possibilities
The FlexLab is the only radiosynthesizer that is able to fully automate all the following synthesis steps:
Step 1:
18F Trapping onto QMA
Step 2:
Reactor 1:
Primary Reaction
Step 3:
S.P.E Purification of Primary Reaction Mixture
Step 4:
HPLC Purification of Primary Reaction Mixture
Step 5:
S.P.E Reformulation of Primary HPLC product
Step 6:
Reactor 2:
Secondary Reaction
Step 7:
S.P.E Purification of Secondary Reaction Mixture
Step 8:
HPLC Purification of Secondary Reaction Mixture
Step 9:
S.P.E Reformulation of Secondary HPLC product
Step 10:
Final Injectable Radiotracer
HPLC Without Limitations
The FlexLab's HPLC system has been designed to give radiochemist's all the tools they need to purify complex compounds multi-step compounds without any compromises.
Dual HPLC Injectors
Allows two independent HPLC purifications in the one synthesis using independent HPLC loops & HPLC columns.
Dual HPLC Column Selector
Automatically select between two different HPLC columns and also provide a column by-pass.
Dual HPLC Product SPE Reformulation
Perform two independent S.P.E. purification of the collected HPLC product (one per HPLC system).
4 x HPLC Eluents
Automatic selection between the 4 eluents.
Fibre Optic UV/VIS Detector
Variable wavelength with a remote fibre optic flow cell.
Quaternary Low Pressure Gradient HPLC Pump
10mL/min pump head (50mL/min option), quad channel degasser and mixer built-in.
HPLC device images: KNAUER Wissenschaftliche Geräte GmbH
Fibre Optic UV Flow Cell
Only the remote fibre optic flow cell is mounted inside the hotcell which frees up valuable space compared to a standard UV detector.
HPLC Low Pressure Gradients
Easily perform fully automated quaternary L.P.G. solvent elutions during your radiosynthesis. All parameters are defined in your recipe file.
Eliminate Manual Solvent Mixing
Quaternary L.P.G. eliminates the need for manual mixing of your HPLC eluent - It's all done on-line.
Flexible Reactors
Easily swap between the standard 20mL reactor and the small volume 2mL reactor.
Open up new possibilities with the quick change small volume reactors by simplifying the transition to peptide labelling.
Reactors available in either standard borosilicate glass or high purity glassy carbon. Heating to 220°C & vortex tube compressed air cooling to ≈ 8°C.
Standard 20mL reactor
Small volume 2mL reactor
LN2 Main Reactor Cooling
Extend the cooling range of both main reactors to -80°C by combining it with our LN2 cooling option.
Quad Reactors
Add unparalleled quad reactor capabilities to the FlexLab. The quad reactor option adds heating (up to 150°C) / cooling to ambient, radioactivity monitoring and magnetic stirrer bar mixing to both intermediate vials.
Compact Dimensions
Install the FlexLab into virtually any hotcell.
.jpg?crc=4252250426)
Width 49.4cm x Height 46.6cm x Depth 41.6cm
Reduce your labs hotcell costs!
No longer do you need a large and expensive research hotcell to house all the equipment needed for complex multi-step synthesis.
The FlexLab easily fits into compact hotcell's with internal dimensions as small as 72cm x 60cm x 60cm (WxHxD) which can substantially reduce your laboratories costs.
Sensors
Complete & precise monitoring.
x10
Radioactivity Detectors
Monitor every step of the synthesis with 10 collimated radioactivity detectors.
CsI(TI) crystal PIN photodiodes provide a linear response across a large radioactivity range.
HPLC Injection Fluid Detectors
Product injection into the HPLC loop's is controlled by two software calibrated optical fluid detectors.
They allow on the fly adjustment of the detection threshold so that you never miss your product injection.
Open User Interface
System control & visual synthesis recipe development all in one platform.
The user interface combines both powerful features, yet easy to use tools for both daily operation and synthesis recipe development.
Easy to use open software interface for easy tracer development
Guides you step-by-step during the synthesis
Can be installed on multiple computers for remote synthesis
Recording of all process variables and report generation (21 CFR Part 11 & GMP compliant)
Traditional PC or Touch Screen Tablet Control
Built-in remote diagnostics enabling simplified troubleshooting
Synthesis Reports
Generate and print synthesis reports to satisfy you labs documentation and GMP requirements
Real-Time Sensors Trends
All sensor information can be displayed graphically in real-time trends
Historical Data Review
Review previous synthesis results as trend graphs with data analysis function
Graphical Synthesis Recipe Development
Click • Define • Save
SAVE STEP
1. Click Schematic
Click schematic elements such as valves, magnetic stirrers & vacuum pump to turn them on/off.
2. Define parameter
Define parameters such as reactor temperatures, HPLC pump parameters (flow rate & eluent composition) & step parameters (description, time, condition).
3. Save Step
Click the Save Step button and the software will automatically fill-in the Excel recipe step list automatically.
Synthesis recipes are stored as easily editable Excel step list files
Feature Comparison
Lets see how the FlexLab compares to other research synthesizers.
FEATURES
Other F-18 Synthesizers
Dual Reactors
Quad Reactors
LN2 Reactor Cooling
Dual Independent HPLC Purification
Quaternary LPG Pump + Degasser
Variable Wavelength Fibre
Optic UV/VIS Detector
Visual Synthesis Recipe Development
Dual SPE HPLC Product Reformulation
*
*
*
* Optional Feature
Example of Compounds that have been Synthesized on the FlexLab
Radiotracer
Imaging
Yield @ E.O.S
(not corrected)
18F-FLT
Proliferation
10%
18F-FLETT (synthon: 18F-FEA)
Proliferation
40%
18F-FMISO
Hypoxia
30%
18F-NaphtoQuinone (synthon:18F-FB)
Hypoxia
10%
18F-FES
Estrogen
20%
18
F-MEL50/54
Melanoma
12%
18F-FDHT
Tumor (androgen receptor expression)
20%
18F-FPPRGD2 (synthon: 18F-NFP)
αvβ3 integrin expression
10%
18
F-GalactoRGD (synthon: 18F-NFP)
αvβ3 integrin expression
10%
18
F-PBR111
Peripheral Benzodiazepine Receptor
30%
1
1
1
1
1
1
3
3
4
2
18F-SFB
18F-FEA
18F-DBCO
18
F-NFP
18F-FB
18F-
FBEM
18F-FP
Synthon
Coupling to free amine of single chain antibody
Peptide (Cu catalysed click chemistry)
Peptide: Cu-free click chemistry
Neovascularisation: coupling to peptide
Coupling to hydroxymethyl napthoquinone
Coupling to sulfhydryl group of modified single chain antibody
Coupling to hydroxmethyl napthoquinone
Peptide
Chemistry
25%
90%
10%
20%
35%
25%
55%
20%
Yield @ E.O.S
(not corrected)
N-Succinimidyl 4-[18F]Fluorobenzoate
2-[18F]Fluoroethylazide
[18F]-Dibenzocyclooctyne (free amine on chain)
4-nitrophenyl 2-[18F]fluoropropionate
[18F]Fluorobenzoic acid
N-[2-(4[18F]Flurobenzamido)Ethyl]
malemide
4-[18F]Fluorobenzaldehyde
2-[18F]Fluoropropionic acid
18F-FPA
Coupling to peptide amines
20%
18F-FBA
1 Ackermann, U., Austin Hospital
2 Australian Nuclear Science & Technology Organisation, Lucas Heights
3 Roselt, P., Peter MacCallum Cancer Centre
4 Bourdier, T., Royal Prince Alfred Hospital
Radiochemical yield not decay corrected
FlexLab Users
Some of the institutions that are using the FlexLab.
...............................................................................
