C-11 Pro2

Complete C-11 radiotracer production lab

C-11 Pro2 radiosynthesizer

The C-11 Pro2 is our next generation synthesizer designed from years of R&D to simplify the routine production of 11C radiotracers.  11CO2 is trapped at room temp. and rapidly released using a volume optimized molecular sieve column.

 

Capable of producing either methyl-iodide or methyl-triflate via the "wet" chemistry method, labelling is performed by traditional reaction vial or room temp. loop labelling* and comes with built-in HPLC purification.

 

The C-11 Pro2 is the complete solution for all your 11C production requirements in the one device.

The C-11 Pro2 has enabled our lab to synthesize an array of more than 10 different 11C compounds, with excellent day-to-day reliability and very good yields and specific activity

Dr. Didier Le Bars

Head of PET Radiochemistry

CERMEP - Imagery Du Vivant, France

DOWNLOAD BROCHURE

Select Your Synthesis Route

The C-11 Pro2 easily allows the selection of the optimal synthesis route.

Step 1:

Concentration of 11CO2 at Room Temperature

Step 2:

Production of Methyl Iodide via Wet Chemistry Method

Step 3A:

Online Conversion of Methyl Iodide into Methyl Triflate

O

O

C

11

O

O

S

O

H

H

H

C

11

C

11

F

F

F

I

H

H

H

C

11

Step 3B:

Reactor Labelling of Precursor with Selected Synthon

Step 3C:

Room Temperature Loop Labelling of Precursor with  Selected Synthon

Step 4:

HPLC Purificaiton

Step 5:

SPE Reformulation of HPLC purified product using a disposable kit

Step 6:

Final Injectable Radiotracer

4 Synthesis options

Optimized radiochemistry

I

H

H

H

C

11

O

O

S

O

H

H

H

C

11

C

11

F

F

F

[11C] Methyl-Iodide

 

[11C]Methyl Iodide is produced in

Reactor 1 via the “wet chemistry”

method and it achieves a > 85%

conversion rate from [11C]carbon

dioxide to [11C]methyl-iodide.

 

[11C] Methyl-Triflate

 

The built-in silver triflate column

allows on-line rapid conversion

of [11C]methyl-iodide into [11C]

methyl-triflate.

 

 

Loop Labelling

 

In addition to the traditional reaction vial

labelling method, the C-11 Pro2 allows

labelling via the room temperature loop

labeling method developed by A. Wilson.

 

 Benefits: Generally less by-products are produced as the reaction is done at room temperature resulting in higher labelling yields.

 

 

 

Reactor Labelling:

 

Reactor 1 is used to generate [11C]methyl-iodide via the "wet chemistry" method.

 

Reactor 2 is used to preform the traditional labelling reaction method with the desired synthon. HPLC loop is loaded with the reaction  mixture using the built-in syringe drive.

 

Reactor 1

Reactor 2

Two disposable 1.1mL tapered bottom glass reactors allow low volume (as low as 75uL reactions and reduces cross-synthesis contaminants which ensures reproducible results.

High Yield & High Specific Activity

No need to compromise -  both can be achieved.

Yield

Specific Activity

Our users have discovered that along with high radiochemical yields, the C-11 Pro2 can achieve high specific activity results as well.

This is due to the C-11 Pro2's design and optimized "wet" chemistry process.

 

 

Specific Activity of compound at EOS

3 - 5 Ci / µmol

111 - 185 GBq / µmol

Example of Compounds that have been Synthesized on the C-11 Pro2

10%

Radiotracer

11C-PIB

11C-Choline

11C-PK11195

11C-Methionine

11C-DASB

11C-Flumazenil

11C-PE2I

11C-Acetate

Imaging

Beta-amyloid plaques in neuronal tissue

Prostate cancer

Peripheral benzodiazepine receptor (PBR)

Primary brain tumors

Serotonin transporter

Benzodiazepine receptor antagoinst (GABAA)

Dopamine transporter (DAT)

Early metastatic prostate cancer

Synthesis Time

33 min

20 min

33 min

20 min

33 min

32 min

34 min

16 min

20%

48%

10%

40%

20%

20%

9%

40%

Yield @ E.O.S

(not corrected)

1,4

1

1

2

3

1

3

3

11C-Raclopride

D2 dopamine receptors

34 min

6

11C-Buprenorphine

11C-UCBJ

11C-metoclopramide

Opioid receptors

(binds to SV2A) measure synapse density

P-glycoprotien function at blood brain barrier

35 min

40 min

5%

7%

5%

7%

11C-diprenorphine

Opioid receptors

40 min

40 min

7

7

7

7

Further compounds synthesized by C-11 Pro users

Radiotracer

11C-CUMI

11C-MePPEP

11C-SCH442,416

11C-RO154513

11C-mHED

Imaging

5-HT(1A) agonist

Cannabinoid CB(1) receptor

Adenosine A2a subtype receptor

a5 subtype-GABA-benzodiazepine receptor

Norepinephrine trasporter (NET)

Synthesis Time

 

 

≈30 min

 

 

≈10-20%

Yield @ E.O.S

(not corrected)

5

1 Austin Hospital, Melbourne Australia

2 Sir Charles Gairdner Hospital, Perth Australia

3 CERMEP - Imagerie Du Vivant, Lyon France

4 Bangkok Hospital Medical Centre, Bangkok Thailand

5 Former Hammersmith Imanet Limited (HIL), London UK

6 Gachon University Hospital, South Korea

7 CEA, France

Radiochemical yield not decay corrected.

Disposable S.P.E. Reformulation Cassette

Simplify GMP requirements

The sterile disposable S.P.E. (solid phase extraction) product reformulation cassette and reagent set help avoid cross-contamination and ensure reproducible results. By using non-proprietary components, the C-11 Pro2 enables users to modify and develop their own cassettes.

 

Disposable Reactors

Two disposable 1.1mL tapered bottom glass reactors allow low volume (as low as 70uL) reactions and reduce cross-synthesis contaminants which ensures reproducible results.

Fully Automated Wash-Up

Immediately post synthesis, the C-11 Pro2 preforms a fully automated wash-up procedure with 3 different solvents to clean all fixed valves and tubing.

Multi-Synthesis Capability

Preform multiple radiosynthesis in one day thanks to the use of disposable components and fully automated synthesizer wash-up.

Save time and and eliminate the hassle by not needing to perform manual cleaning procedures with the C-11 Pro2.

Compact Dimensions

Install the C-11 Pro2 into virtually any hotcell.

Width 51cm  x  Height 43.4cm  x  Depth 34cm

No longer do you need a large and expensive research hotcell to house all the equipment needed for the complete synthesis of C-11 compounds.

 

The C-11 Pro2 can fit into compact hotcells which reduces your labs hotcell costs.

Compact HPLC System

The C-11 Pro2's compact HPLC contains all the features needed to purify compounds.

HPLC Pump

50mL/min pump head with integrated pressure sensor.

Fibre Optic UV/VIS Detector

Variable wavelength and fibre optic connection to the flow cell.

Only the remote fibre optic flow cell is mounted inside the hotcell which frees up valuable hotcell space compared to traditional UV detectors.

HPLC device images: KNAUER Wissenschaftliche Geräte GmbH

User Interface

System control & synthesis recipe development all in one.

C-11 Pro2 software interface

The user interface combines both powerful features, yet easy to use tools for both daily operation and synthesis recipe development.

Easy to use open software interface for easy tracer development

Guides you step-by-step during the synthesis

Can be installed on multiple computers for remote synthesis

Recording of all process variables and report generation (21 CFR Part 11 & GMP compliant)

Traditional PC or Touch Screen Tablet Control

Built-in remote diagnostics enabling simplified troubleshooting

Synthesis Reports

Generate and print synthesis reports to satisfy you labs documentation and GMP requirements

Real-Time Sensors Trends

All sensor information can be displayed graphically in real-time trends

Historical Data Review

Review previous synthesis results as trend graphs with data analysis function

Graphical Synthesis Recipe Development

Click • Define • Save

SAVE STEP

1. Click Schematic

Click schematic elements such as solenoid valves, rotary valves & rotary actuators to turn them on/off or to set positions.

2. Define parameter

Define parameters such as reactor temperatures, MFC flow, syringe / needle positions, HPLC pump parameters & step parameters (description, time, condition).

3. Save Step

Click the Save Step button and the software will automatically fill-in the Excel recipe step list automatically.

Synthesis recipes are stored as easily editable Excel step list files

Feature Comparison

Lets see how the C-11 Pro2 compares to other synthesizers.

Other C-11 Synthesizers

FEATURES

[11C]Methyl Iodide Yield

[11C]Methyl Triflate Conversion

Reactor Labelling

Loop Labelling

Variable Wavelength Fiber

Optic UV/VIS Detector

Visual Synthesis Recipe Development

Disposable SPE Cassette

*

>85%

>50%

* Optional Feature

C-11 Pro Users

Some of the institutions that are using the C-11 Pro.

iPHASE Support?

Key aspects of our support structure

Spare Parts

Complete stock of spare parts for all synthesizers are available and can be expressed shipped to you to minimise downtime.

Continuing Education

The field of radiochemistry is

ever changing and our education program is there to ensure your team is fully up to date with our latest developments.

Technical Support

You are always  a phone call or an email away from an experienced iPHASE engineer or staff member to assist with any queries.  Achieving customer satisfaction is our primary objective.

Upgrades

Due to the ever evolving nature of technology, we are continually  following the trends and applying the latest technologies to our systems to increase performance, productivity and reliability.

Remote diagnostics

Remote diagnostics are built into every system we make. This enables our experienced engineers to diagnose, test and guide the user to the quickest solution to an issue should it arise.

Hands on Training

Personalized hands on training will ensure your staff will easily learn and master all aspects of our automated technology.

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